Generator

Part:BBa_K1061021:Design

Designed by: mengyi sun   Group: iGEM13_SYSU-China   (2013-09-17)


EF-1alpha-apoptin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 715
    Illegal XhoI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 440
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although it has been try in a broad spectrum of cells and prove to be safe to the normal cells, it still has not been tried in ALL normal cells. So before using it to treat certain kinds of cancer, every team need to ensure that it want hurt the specify normal cells involve in the project. And it is a protein contain 121 amino acid that may big enough to induce immune reaction(although in the experiment of mouse it has not been reported.)


Source

We got the apoptin from Zhuang's lab,and cloned the EF-1alpha promoter from the plasmid provided by Lei Xiao.

References